北京师范大学学报：自然科学版2012，Vol.48Issue(1)：47-53,7.
拟南芥转or基因突变体转录组及表型分析
TRANSCRIPTOME AND PHENOTYPE ANALYSES OF or GENE TRANSGENIC ARABIDOPSIS MUTANT
摘要
Abstract
It is known that orange（or） gene mutation leads to increased carotenoids content in plants.To elucidate the detailed mechanisms involved,Arabidopsis plants with over-expressed or and vector-only control were generated.Pigment content,transcriptome profile and mutant phenotypes were investigated.It was found that or was functional in green tissues,such as leaves and stems,although less functional than in non-green tissues.No major changes in transcription pattern were found for genes involved in carotenoids biosynthesis,but resistance-related genes,such as ZAT,were up-regulated,implying stressed environment in or mutants.Hypocotyl length measurements under different light conditions suggested that the or mutant became sensitive to light especially to blue light.Carotenoids enhancement of or in different plant tissues was analyzed,providing a reference for genetic engineering using or to improve nutritional status of crops,and for future work to uncover or regulatory mechanisms.关键词
or基因/类胡萝卜素/转录组/胁迫/光表型Key words
or gene/carotenoids/transcriptome/stress/photic phenotype分类
农业科学引用本文复制引用
门潇,孙天虎,杨永华..拟南芥转or基因突变体转录组及表型分析[J].北京师范大学学报：自然科学版,2012,48(1):47-53,7.基金项目
国家自然科学基金资助项目 （）
