北京大学学报（医学版）2012，Vol.44Issue(2)：195-198,4.DOI:10.3969/j.issn.1671-167X.2012.02.007
抗鼠科肉瘤病毒癌基因同源物B1抗体的检测及其在类风湿关节炎诊断中的意义
Significance of the detection of anti-v-raf murine sarcoma viral oncogene homologue B1 antibodies in the diagnostic practice of rheumatoid arthritis
摘要
Abstract
Objective: To clarify the clinical significance of the antibody against v-raf murine sarcoma viral oncogene homologue Bl ( BRAF) in the diagnostic practice of rheumatoid arthritis (RA). Methods: In the study, 112 patients with RA, 112 patients with other rheumatic diseases,and 73 healthy individuals were recruited . With recombinant human BRAF protein as antigen, we examined the level of anti-BRAF antibody in all the patients by enzyme-linked immunosorbent assay ( ELISA) , The clinical data of the RA patients were collected simultaneously, and analysed statistically by using SPSS 13. 0. Results: The positive rate of anti-BRAF antibody was 53. 6% in the RA patients, which was significantly higher than that of the normal control group(4. 1% ,P <0. 01 ) and other rheumatic diseases groups (P all <0. 01) except osteoarthritis group. The titer of anti-BRAF antibody was also notably higher in the patients with RA than in other rheumatic diseases and normal control groups(P all <0. 01). The diagnostic sensitivity and specificity of anti-BRAF antibody for RA were 53. 6% and 84. 3% respectively. The positive rate of anti-BRAF antibody in rheumatoid factor, anti-cyclic citrullinated peptide antibody, antikera-tin antibody,antiperinuclear factor negative groups were 52. 6% ,38. 2% , 30. 3% and 31.0% respectively. It showed significant negative correlation between the titer of anti-BRAF antibody and patient' s age, disease duration and the level of CRP. Conclusion: The anti-BRAF antibody contributes to the diagnosis of RA, and may act as a supplement of other autoantibodies.关键词
关节炎,类风湿/抗鼠科肉瘤病毒癌基因同源物B1/酶联免疫吸附测定Key words
Arthritis, rheumatoid/ v-raf murine sarcoma viral oncogene homologue Bl/ Enzyme-linked immunosorbent assay分类
医药卫生引用本文复制引用
张霞,赵金霞,孙琳,李茹,刘湘源,栗占国..抗鼠科肉瘤病毒癌基因同源物B1抗体的检测及其在类风湿关节炎诊断中的意义[J].北京大学学报（医学版）,2012,44(2):195-198,4.基金项目
国家自然科学基金(81030057,81072474)和北京市自然科学基金(7112143)资助 （81030057,81072474）
