北京大学学报（医学版）2012，Vol.44Issue(2)：240-243,4.DOI:10.3969/j.issn.1671-167X.2012.02.017
原发性干燥综合征合并肺间质病变患者血清特异性标志物的筛选
Screening for serum specific biomarkers in patients with primary Sj(o)gren' s syndrome and interstitial lung disease using proteomic fingerprint techniques
摘要
Abstract
Objective;To detect the serum protein biomarkers and establish a diagnostic model for primary Sjogren' s syndrome (pSS) with interstitial lung disease (ILD). Methods; Serum samples from 69 patients with pSS were prepared with WCX magnetic beads, and analyzed on PBS II -C mass spectrometer reader. Biomarker Wizard software was used to detect protein peaks and potential difference between the patients with pSS-ILD and with non-ILD. The model was developed by Biomarker Patterns software. Results; Totally 7 discriminative mass-to-charge (m/z) ratios were identified to be related with pSS-ILD (P <0. 05). Among these, the m/z peaks at 3 778. 3, 3 318. 3 and 2 236.6 were used to construct a diagnostic model. The sensitivity and specificity of the model were 93. 1% and 87. 5% , respectively. In a testing set, the sensitivity and specificity of the model were 84.0% and 85.7% , respectively. Conclusion; The potential protein biomarkers for pSS-ILD are discovered in the serum by MALDI-TOF-MS combined with WCX magnetic beads. The diagnostic pattern combining 3 778.3, 3 318.3 and 2 236.6 m/z protein peaks can discriminate pSS-ILD and non-ILD.关键词
干燥综合征/肺疾病,间质性/蛋白质组学Key words
Sjogren' s syndrome/ Lung disease, interstitial/ Proteomics分类
医药卫生引用本文复制引用
李玉慧,孙晓麟,何菁,贾汝琳,杨冬月,张学武,栗占国..原发性干燥综合征合并肺间质病变患者血清特异性标志物的筛选[J].北京大学学报（医学版）,2012,44(2):240-243,4.基金项目
首都医学发展科研基金(2009-2030)和北京市自然科学基金(7102155)资助 （2009-2030）
