草业科学2012，Vol.29Issue(3)：417-421,5.
油茶SOD基因片段克隆及序列分析
Cloning and sequence analysis of SOD gene of Camellia oleifera
摘要
Abstract
Two pairs of degenerate primers were designed based on the conservative regions in amino acids of Cu/Zn-SOD and Mn/Fe-SOD genes from other plants,respectively.Cu/Zn-SOD gene fragment and Mn/Fe-SOD gene fragment of Camellia oleifera were cloned.Their sizes were 375 bp and 429 bp,respectively.By using the program of BLAST on NCBI GenBank database,two sequences presented a very high identity with Cu/Zn-SOD and Mn/Fe-SOD genes from other plants.The nucleotide sequences were analyzed by using program of DNAStar 5.0.125 amino acids and 143 amino acids were coded by the nucleotide sequences and the nucleotide sequences had the same conservative regions of Cu/Zn-SOD and Mn/Fe-SOD genes as many kinds of other plants.The homology comparison of nucleotide sequences of Cu/Zn-SOD gene and Mn/Fe-SOD gene showed the similarities of more than 80% and 84% to other plants,respectively.The similarities of amino acid sequences of Cu/Zn-SOD and Mn/Fe-SOD genes were more than 84% and 86% to other plants.关键词
油茶/SOD/基因克隆/序列分析Key words
Camellia oleifera/SOD/gene cloning/sequences analysis分类
农业科技引用本文复制引用
郭春兰,张露..油茶SOD基因片段克隆及序列分析[J].草业科学,2012,29(3):417-421,5.基金项目
基金项目：国家科技部“十一五”科技支撑项目 （）
