草业学报2012,Vol.21Issue(2):116-122,7.
早春短命植物绵果荠愈伤组织诱导相关因素研究
The relevant factors of callus induction of early spring ephemeral Lachnoloma lehmannii (Brassicaceae)
摘要
Abstract
Lachnoloma lehmannii is an annual early spring ephemeral Brassicaceae species in the Junggar Desert of northern Xinjiang.The primary aims of this study were to research the conditions for callus induction in L.lehmannii.Using the aspect seedling cotyledon as explants,the effect of seedling age,media,sucrose concentration and the culture condition on callus induction and their growth was investigated.The most suitable condition for callus induction was seedlings of optimal age (14 days) on an MS culture medium containing 1.0 mg/L IBA+0.1 mg/L BAP,with a suitable concentration of sucrose (3%),at a temperature of 25°C in the dark.The best condition for callus growth was a temperature of 25℃ in the light with a photoperiod of 16 h/day.关键词
早春短命植物/绵果荠/愈伤组织Key words
early spring ephemeral/Lachnoloma lehmannii/callus分类
农业科技引用本文复制引用
曼苏尔·那斯尔,谭敦炎,廖康..早春短命植物绵果荠愈伤组织诱导相关因素研究[J].草业学报,2012,21(2):116-122,7.基金项目
新疆维吾尔自治区高技术研究与发展计划项目(200810102)资助. (200810102)