草业学报2012，Vol.21Issue(2)：133-140,8.
利用SCoT标记分析不同秋眠型苜蓿的遗传多样性
Optimization of SCoT reaction system and genetic diversity of different fall dormancy alfalfa
摘要
Abstract
Genomic DNA was extracted by advanced CTAB methods from Medicago sativa and Medicago his pi-da.The optimum SCoT-PCR (start codon targeted polymorphism) reaction system in alfalfa was established with orthogonal design experiments on four levels of five factors (DNA,dNTPs,Mg2+,Taq polymerase,and primer).A suitable SCoT reaction system was a 20 μL mixture containing 50 ng DNA,1.00 mmol/L Mg2+,1.20 U Taq polymerase,0.40 mmol/L dNTPs and 0.30 μmol/L primers.Of the 50 primers screened,13 generated highly polymorphic fragments useable as SCoT markers,and 34 accessions were amplified by these 13 primers.A total of 103 bands (including 92 polymorphic bands) were detected by the 13 chosen primers.The 34 accessions were divided into two groups.Two M.hispida cultivars from Anhui and Jiangsu were clustered into one group.The remaining 32 cultivars were classified into three sub-groups with a similarity coefficient of 0.757,Fall dormancy alfalfa cultivars were mostly distributed in the second sub-group.Semi-fall dormancy and non-fall dormancy alfalfa cultivars did not converge into the same sub-group thus showing incomplete similarity between fall dormancy alfalfas.关键词
苜蓿/SCoT/条件优化/秋眠型/遗传多样性Key words
alfalfa (Medicago spp.)/SCoT/condition optimization/fall dormancy/genetic diversity分类
农业科技引用本文复制引用
何庆元,王吴斌,杨红燕,向仕华,周丽英,王松华..利用SCoT标记分析不同秋眠型苜蓿的遗传多样性[J].草业学报,2012,21(2):133-140,8.基金项目
国家"973"计划基金项目(2007CB108901),安徽省教育厅重大基金项目(ZD200910)和安徽科技学院校级重点学科(AKXK20l02-1)资助. （2007CB108901）
