草业学报2012，Vol.21Issue(2)：149-155,7.
转红树总DNA紫花苜蓿T1代耐盐株系的生理生化特性分析
Analysis of physiological and biochemical characteristics of T1 salt-tolerant transgenic alfalfa transformed with the Rhizophora apiculatatotal total DNA via the pollen-tube pathway
摘要
Abstract
With 300 mmol/L NaCl stress,the physiological and biochemical indexes,including chlorophyll,proline and MDA content,and antioxidase activity,of T1 transgenic alfalfa transformed with Rhizophora apic-ulata total DNA by the pollen-tube pathway,were investigated.The transgenic plantlets had a reduced chlorophyll content of 0.09 -0.29 times,but the non-transgenic plant had 0.32 times.The proline content of transgenic plantlets was enhanced by 1.2 to 4.2 times,but for non-transgenic plant it was 0.9 times.The increased proportion of MDA content was 0.15 -0.29 times in transgenic plantlets compared with 0.36 times for non-transgenic plant.However,the transgenic plantlets had increased SOD activity (0.20-0.25 times) compared with the non-transgenic plant (0.19 times).The POD and CAT activity of the transgenic plantlets were 1.6-5.6 times and 1.2 -1.3 times respectively compared with non- transgenic plants.This suggested that the transgenic plantlets had the physiological and biochemical characteristics of salt tolerant plants.关键词
紫花苜蓿/花粉管通道法/红树总DNA/T1代株系/耐盐Key words
alfalfa (Medicago sativa)/pollen-tube pathway/Rhizophora a piculata total DN A/T1 transgenic plantlets/salt-tolerance分类
农业科技引用本文复制引用
张立全,敖登花,师文贵,牛一丁,韩岚,哈斯阿古拉..转红树总DNA紫花苜蓿T1代耐盐株系的生理生化特性分析[J].草业学报,2012,21(2):149-155,7.基金项目
国家科技支撑计划子课题(2008BADB3B01-02),内蒙古自然科学基金重大项目(2011ZD03),内蒙古大学"513人才计划"科研项目和内蒙古大学高层次引进人才科研启动基金项目(115106)资助. （2008BADB3B01-02）
