传感技术学报2012,Vol.25Issue(2):151-156,6.DOI:10.3969/j.issn.1004-1699.2012.02.001
食物致病菌快速检测系统的设计与实现
Design and Realization of a Rapid Food Pathogen Detection System
摘要
Abstract
Based on the principle of antigen-antibody and microbial immunosensor using TiO2 nanowire bundle as sensitive material, a rapid food pathogen detection integrated system is designed and realized. The impedance detection circuit adopts a highly integrated chip AD5933 to measure TiO2 nanowire bundle impedance variation with method of electrochemical impedance spectroscopy. E. Coli are used as an experimental sample for testing the performance of detection integrated system. The system can rapid detect the E. Coli in food in one hour, and the detection limit can reach 4. 5x102 cfu/mL. The immunosensor of system shows excellent reproducibility between frequency of 1000 Hz ~ 10000 Hz.关键词
食品安全/食物致病菌检测/电化学阻抗谱/TiO2纳米线/免疫传感器/AD5933Key words
food safety/food pathogen detection/electrochemical impedance spectroscopy/TiO2 nanowire/immunosensor/AD5933分类
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许丽,董文钧,王学斌,陈本永..食物致病菌快速检测系统的设计与实现[J].传感技术学报,2012,25(2):151-156,6.基金项目
项目来源:浙江省科技计划项目(2008C23011) (2008C23011)
浙江省高等学校创新团队项目(T200909) (T200909)
国家自然科学基金项目(51005213) (51005213)