传感技术学报2012,Vol.25Issue(3):319-321,3.DOI:10.3969/j.issn.1004-1699.2012.03.007
基于MEMS微电极芯片的电穿孔系统
Electroporation System Based on MEMS Micro-Electrode Chip
摘要
Abstract
We demanstrated an cell electroporation system based on Au electrode microfabricated chip. We realized high efficiency electroporation in both standard expression cell( HEK-293A)and hard-to-transfected cell(3T3-Ll) , with a transfection rate of 90% and 80% .respectively. The operation system is friendly,With manual mode and auto mode. The unique electrode design promises much low applied voltage, low cost and easy operation.关键词
生物MEMS/细胞电穿孔/微加工/微型电穿孔系统/低电压Key words
bio-MEMS/cell electroporation/micro fabrication/electroporation system/low voltage分类
信息技术与安全科学引用本文复制引用
闫浩,魏泽文,李学明,赵德尧,梁子才,李志宏..基于MEMS微电极芯片的电穿孔系统[J].传感技术学报,2012,25(3):319-321,3.基金项目
国家自然科学基金项目(61176111) (61176111)