传感技术学报2012，Vol.25Issue(4)：423-427,5.DOI:10.3969/j.issn.1004-1699.2012.04.001
基于蛋白A-纳米金-丝网印刷电极的雌二醇免疫传感器
An Immunosensor for Detection of 17β-Estradiol Based on Screen-Printed Electrode with Nano-Gold and Protein-A
摘要
Abstract
A immunosensor for detection of 17β-estradiol was prepared by modifying nano-gold and protein-A on the surface of screen-printed electrode. The large specific surface area and high surface free energy of gold nanopaticles could make protein-A molecules immobilized firmly,and then protein-A was used to directionally immobilize the Fc portions of anti-17|$-estradiol antibody. Thus a immunosensor was developed for rapid detection of 17β-estradiol by competitive enzyme-linked immunoassay. The modified process was characterized by scanning electron microscopy (SEM)and cyclic votammetry ( CV). Under the optimized conditions, the immunosensor showed high sensitivity. There was a negative linear correlation between the current of reduction peak and the concentration of 17β-estradiol in the range of 0.1 u.g/L -20 u,g/L with a detection limit of 0.035 ug/L( S/N =3). When this immunosensor was used to determine the real normal human urine samples, the results were consistent well with high performance liquid chromatography method,and the recovery was in the range of 96% -114%.关键词
免疫传感器/纳米金/蛋白A/17β-雌二醇/丝网印刷电极Key words
immunosensor/nano-gold/protein-A/ 17P-estradiol/screen-printed electrode分类
信息技术与安全科学引用本文复制引用
杨勇,牟婧男,丁亚萍,王俊霞,李健爽,杜晓燕,常东..基于蛋白A-纳米金-丝网印刷电极的雌二醇免疫传感器[J].传感技术学报,2012,25(4):423-427,5.基金项目
国家自然科学基金项目(30972488) （30972488）
