材料科学与工程学报2011，Vol.29Issue(6)：911-915,897,6.
骨形成蛋白-2转染MG-63细胞增加其对壳聚糖膜的粘附
Adhesion of Bone Morphogenetic Protein-2-transfected MG 63 Cells to Chitosan Membranes
摘要
Abstract
To evaluate the adhesion and proliferation of bone morphogenetic protein-2（BMP-2）-transfected MG 63 cells,recombinant bone morphogenetic protein-2（BMP-2） plasmids were constructed and transfected into MG 63 cells.The transfection was analyzed and confirmed by pulimerase chain reaction（PCR）,Western bloting,and cell immunohistochemisty.Chitosan membranes（CSs） were prepared,on which BMP-2-transfected MG 63 cells were cultured.The adhesion between the cells and the membranes was analyzed with scanning electron microscopy（SEM） in d1,d3,and d5,and proliferation of the cells was assessed with（3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazolium）,（MTS） assay in 24h,48h,72h,and 96h.The recombinant BMP-2 plasmid DNA was successfully constructed and transfected into the MG63 cells.SEM analysis indicated that significant increase of adhesion occurred in d1,d3,and d5 compared with the control,while MTS showed no significant difference in cell proliferation.BMP-2-transfected MG63 cells significantly increased their adhesion to chitosan membranes,which may have clinical application in bone rebuilding.关键词
骨形成蛋白/壳聚糖/成骨样细胞Key words
bone morphogenetic protein/chitosan membrane/MG 63 cell/adhesive/proliferation分类
医药卫生引用本文复制引用
范丽,卢岩,林慧平,姚航平,林军..骨形成蛋白-2转染MG-63细胞增加其对壳聚糖膜的粘附[J].材料科学与工程学报,2011,29(6):911-915,897,6.基金项目
浙江省自然基金资助项目 （）
