重庆医学2012，Vol.41Issue(15)：1462-1464,封3,4.DOI:10.3969/j.issn.1671-8348.2012.15.003
左金丸醇提物抑制幽门螺旋杆菌感染人胃癌细胞增殖及诱导凋亡的实验研究
Experimental study on inhibiting proliferation and inducing apoptosis of Zuojinwan alcohol extracts on human gastric cancer cells infected by Helicobacter pylori
摘要
Abstract
Objective To study the influence of Zuojinwan alcohol extracts on proliferation and apoptosis of human gastric cancer cells MKN45 infected by Helicobacter pylori(H. Pylori) and to investigate their mechanism. Methods The different concentrations of Zuojinwan alcohol extracts (7. 81,15. 63,31.25,62. 5,125,250,500 μg/mL) acted on human gastric cancer cells MKN4 5 infected by H. Pylori respectively. After 24,48,72 h,the cell proliferation activity and apoptosis were detected by MTT and FCM respectively,and the expression of Bax and PARP was detected by Western blot. Results FCM results showed that the percentage of apoptotic cells of MKN45 cells infected by H. Pylori,Zuojinwan alcohol extracts low dose (40 μg/mL) and Zuojinwan alcohol extracts high dose(180 μg/mL) were (0. 28 + 0. 105)% ,(3. 27 + 0. 702)% and (7. 7 + 0. 721)%, with statistical difference between any two groups(P<0. 05). The apoptosis rate was dose-dependent. Western blot results showed that after 24 h action of high and low doses of Zuojinwan alcohol extracts on MKN45 cells, the expression of PARPC89 KD) and Bax protein was significantly increased in a dose-dependent manner. Conclusion Zuojinwan alcohol extracts can inhibit the proliferation of human gastric cancer cells MKN45 infected by H. Pylori and induce apoptosis. Regulation of apoptosis-related genes Bax and PARP expression may be one of the molecular mechanisms for inducing apoptosis of human gastric cancer cell MKN45 infected by H. Pylori.关键词
左金丸醇提物/幽门螺旋杆菌/胃癌细胞/增殖/凋亡Key words
Zuojinwan alcohol extracts/H. Pylor/human gastric cancer cell/proliferation/apoptosis引用本文复制引用
汤庆丰,刘宣,葛艳,周宁,周利红,孙健,季青,高虹,李琦..左金丸醇提物抑制幽门螺旋杆菌感染人胃癌细胞增殖及诱导凋亡的实验研究[J].重庆医学,2012,41(15):1462-1464,封3,4.基金项目
国家自然科学基金资助项目(81072955) （81072955）
上海市卫生局科研基金资助项目(2010019) （2010019）
上海市卫生局青年基金资助项目(2011001) （2011001）
上海市重点学科基金资助项目(S30302). （S30302）
