摘要
Abstract
Objective To explore the expression of anti-survivin antibody and survivin mRNA in peripheral blood of patients with colorectal cancer and its clinical significance. Methods 116 patients with colorectal cancer were divided into radical surgery group, palliative surgery group and palliative chemotherapy group, and 30 healthy people were taken as controls. Peripheral blood was collected before and after treatment, and the expression levels of anti-survivin antibody and survivin mRNAwere detected by ELISA and semi-quantitative RT-PCR respectively. Results The positive rates of anti-survivin antibody were respectively 40. 5% and 0,and survivin mRNAwere respectively 76. 7% and 0,in peripheral blood of patients with colorectal cancer and healthy people, and differences were significant(P = O. 000). The expression levels of anti-survivin antibody and survivin mRNA increased with the advance of tumor stage,tumors > 5cm in diameter were significantly higher than those 5^5 cm(P = 0. 045,P = 0. 003) ,those with lymph node metastasis were significantly higher than those without lymph node metastasis(P = 0. 040,P = 0. 000) ,those after radical surgery were significantly lower than those preoperation(P = 0. 005,P = 0. 004) ,those with a high expression were significantly higher in metastatic rate than those with a low expression after radical resection(P = 0. 001,P = 0. 004). There was no significant difference of expression levels between before and after palliative surgery(P = 0. 709 , P = 0. 669). In palliative chemotherapy groups, the expression levels after chemotherapy were significantly lower than before chemotherapy in the effective group (P = 0. 04 3,P = 0. 032) ,anti-survivin antibody was no significant change(P = 0. 135) ,but survivin mRNAexpression in those after chemotherapy was significantly higher than those before chemotherapy in the ineffective group(P = 0. 039). Conclusion The detection of anti-survivin antibody and survivin mRNA in peripheral blood may have certain clinical significance to the diagnosis,prognostic estimation, efficacy prediction of chemotherapy,and follow-up of colorectal cancer.关键词
结直肠肿瘤/诊断/survivinKey words
olorectal neoplasms/diagnosis/ survivin
