重庆医学2012，Vol.41Issue(16)：1614-1616,封4,4.DOI:10.3969/j.issn.1671-8348.2012.16.020
培美曲塞对SKP2蛋白表达的影响及意义
Impact of Pemetrexed on expression of SKP2 in adenocarcinoma of lung cell lines A549 cellandits significance
万琴 1王顺金1
作者信息
- 1. 南昌大学附属第二医院肿瘤科,江西南昌,330006
- 折叠
摘要
Abstract
Objective To investigate the expression variation and significance of s-phase kinase-associated protein 2CSKP2) in lung adenocarcinoma A549 cells after the treatment of Pemetrexed(PMX). Methods On the basis of the concentration of PMX, we set one control group and four experimental groups :OjlaM( group A) ,0. OljuMCgroup B),0. ljuMCgroup C) , ljuMCgroup D) , IOjlaM (group E). The growth of A549 was tested by MTT assay. The effect of PMX on the cell cycle phase distribution of A549 was analyzed by flow cytometry. The expression of SKP2 protein of A54 9 cells was detected by Western blot. Results MTT assay showed that PMX could significantly inhibit the growth of A549 cells,and the highest inhibitory rate was(78. 61 + 3. 66)% at the concentration of IOjlaM when A549 cells were cultured for 72 h(P<0. 01). Meanwhile, S-phase cells in the group C,D and E was more than those in the group A(P<0. 05 ,P<0. 01 ,P<0. 01). The difference of SKP protein expression among various groups was sig-nificantCP^O. 01) ,and compared with the control group, SKP2 protein expression was significantly increased in the experimental groups(P<0. 01). Conclusion PMX could inhibit the growth of A549 cells and arrest them in S-phase. The expression of SKP2 protein in PMX-treated A549 cells is upregulated,which might influence the chemotherapy effect of PMX.关键词
肺肿瘤/细胞周期/S期激酶相关蛋白质类/培美曲塞Key words
lung neoplasms/cell cycle/ S-phase kinase-associated proteins/ pemertrexed引用本文复制引用
万琴,王顺金..培美曲塞对SKP2蛋白表达的影响及意义[J].重庆医学,2012,41(16):1614-1616,封4,4.
