重庆医学2012，Vol.41Issue(17)：1680-1683,4.DOI:10.3969/j.issn.1671-8348.2012.17.004
转录因子AP-2α对HeLa细胞内14-3-3σ表达的影响
Effect of transcription factor AP-2α on expression of 14-3-3α in HeLa cells
摘要
Abstract
Objective To investigate the effect of AP-2a on thel4-3-3σ expression in HeLa cells. Methods AP-2α was overex-pressed or RNA interfered in HeLa cells. The quantitative RT-PCR and Western blot were performed to examine the expression of AP-2α and 14-3-3σ. The transcription factor binding sites in the 14-3-3σ promoter were predicted by using the computer software. Dual-luciferase reporter assay was used to test the effect of AP-2α on the transcriptional activity of 14-3-3σ promoter. Results Overexpression of AP-2α led to the increased mRNA and protein levels of 14-3-3σ,and the mRNA and protein expression of 14-3-3σ was decreased when AP-2α was interfered by siRNA in HeLa cells. The results from the computer software analysis showed the multiple potential AP-2α binding sites in 14-3-3σ promoter. Dual-luciferase reporter assay confirmed that AP-2α enhanced the transcriptional activity of 14-3-3σ promoter. Conclusion AP-2α positively regulates the 14-3-3σ expression in HeLa cells probably through enhancing the transcriptional activity of 14-3-3σ promoter.关键词
AP-2/14-3-3σ/HeLa细胞/转录因子Key words
AP-2 / 14-3-3σ/ HeLa cells/ transcription factor引用本文复制引用
甘露,黄明元,蒙子君,张哲,罗炳德..转录因子AP-2α对HeLa细胞内14-3-3σ表达的影响[J].重庆医学,2012,41(17):1680-1683,4.基金项目
国家自然科学基金资助项目(31070687) （31070687）
广东省自然科学基金资助项目(9451051501002535). （9451051501002535）
