重庆医学2012，Vol.41Issue(17)：1719-1721,1726,4.DOI:10.3969/j.issn.1671-8348.2012.17.018
DNA甲基化数据分析方法和软件应用
Data analysis and its analytical softs application on DNA methylation in tumor research
摘要
Abstract
Objective To analyze the quality control method,data analytic key points, results confirmation and data visualization processing during the experimental process of DNA methylation chip. Methods We used published paper and our original research data to explore the methods used in DNA methylation analysis. Results The quality control includes DNA segmentation, positive control selection,noise signal removing and data normalization. Mehtylation specific PCR(MSP) and bisulfite sequencing PCR(BSP) were needed in validation of MeDIP-Chip array results. Methprimer and Methyl Primer Kxpress were used for primer designing. MeDIP-Chip array results were visualized by signal map and BSP result could be analyzed by QUMA and BISMAConclusion In DNA methyaltion research,multifactorial quality control in MeDIP-chip,design and data analysis is necessary.关键词
DNA甲基化/软件/质量控制/数据分析Key words
DNA methylation/ software/ data analysis/ quality control引用本文复制引用
付利娟,夏映曦,何俊琳,刘学庆,陈雪梅,王应雄,丁裕斌..DNA甲基化数据分析方法和软件应用[J].重庆医学,2012,41(17):1719-1721,1726,4.基金项目
重庆市生殖健康与出生缺陷重点实验室开放课题(0801) （0801）
重庆市科委项目(CSCT2009BB5271). （CSCT2009BB5271）
