重庆医学2012，Vol.41Issue(18)：1792-1794,1798,4.DOI:10.3969/j.issn.1671-8348.2012.18.003
3种不同类型基因导入系统转染类神经元细胞的效率比较
The comparison of the transfection efficiency of three different types of gene expression system in the neurotypic cell lines
摘要
Abstract
Objective Use three different types of gene expression system carry the Paav-EGFP plasmid can express green flu-orescent protein to transfect the neurotypic cell lines and to compare their cell toxicity and their transfection effects. Methods We used the commercial liposomes and nanoparticles such as the LipofectAMINE 2000,the PEI(25×103) and PEG-PEI 4. 6 as the gene expression system, carry the Paav-EGFP plasmid to transfect the commonly used neurotypic cells in nervous system cells such as the SH-SY5Y neuroblastoma cells and C6 glioma cells. Then we detected the cell toxicity through the MTT method,and detected the transfection effects by the inverted microscope fluorescent observation and the flow cytometric method. Results MTT detection results showed the cell survival rate of PEG-PEI 4. 6 (83%)was higher than PEI(70%)and LipofectAMINE 2000(73%) in the C6 glioma cells (P<0. 05) ,but the compare of the cell survival rate of PEI and LipofectAMINE 2000 had no statistical sig-nificance(P>0. 05) ;The similar results were in the SH-SY5Y neuroblastoma cells. The inverted microscope fluorescent observed in C6 glioma cells the PEG-PEI 4. 6 had higher fluorescence expression than LipofectAMINE2000 and PEI. While in SH-SY5Y neuroblastoma cells,the LipofectAMINE2000 had higher fluorescence expression than other two materials. At the same time, the PEG-PEI 4. 6 had higher fluorescence expression than PEI. The flow cytometric results showed in C6 glioma cells the PEG-PEI 4. 6 had the best efficiency to 23. 2% , LipofectAMINE2000 was next of 16. 9% ,the worst PEI was 12. 6% (P<0. 05) ; In SH-SY5Y neuroblastoma cells LipofectAMINE2000 had the best efficiency,PEG-PEI 4. 6 second,and PEI was the worst (P<0. 05) ,the efficiency of them was 22. 3% , 17. 2% and 10. 6% respectively. Conclusion At present LipofectAMINE 2000 and PEG-PEI 4. 6 can serve as the gene transfection carrier of the nervous system.关键词
神经胶质瘤/神经母细胞瘤/脂质体/转染Key words
glioma/neuroblastoma/liposomes/transfection引用本文复制引用
梁兵,袁芳,杨宁,殷建瑞,蒲蜀湘,解龙昌,高庆春,高聪..3种不同类型基因导入系统转染类神经元细胞的效率比较[J].重庆医学,2012,41(18):1792-1794,1798,4.基金项目
国家自然科学基金青年项目(81100890) （81100890）
广东省自然科学基金资助项目(10151018201000022) （10151018201000022）
广东省博士启动项目(10451018201004383) （10451018201004383）
广州市属高校羊城学者科研项目(10A010G) （10A010G）
广州医学院博士启动基金(2008c46). （2008c46）
