结直肠肛门外科2012，Vol.18Issue(2)：125-127,3.
脾细胞移植诱导大鼠小肠移植微嵌合体形成的实验研究
The formation of chimerism in rat small bowel transplantation by splenocyte infusion
摘要
Abstract
Objective To study the relationship between chimerism induced by infusing donor splenocyte and immunologic tolerance of small bowel transplantation in rat. Methods The prepared donor splenocytes were infused into recipient via lingual vein four weeks before establishment of allogeneic segmental small bowel transplantation heterotopically in rats, the specific DNA fragment of Y chromosome was detect in peripheral blood and skin by Polymerase Chain Reaction technique after operation. The relationship between chimerism and acute rejection was evaluated by histopathology examination. Results The survival time after small bowel trans-plantation was 7. 1 + 1. 2 d in control group, 18. 4 + 3. 6 d in experimental group. The survival time was significant longer ( P <0. 05) in experimental group than in control group. The chimerism in the experimental group is higher than control group. The acute rejection in the group with positive chimerism is mild than that of negative. Conclusion The infusion of donor splenocyte pro-motes the formation of chimerism and chimerism may induces specific immunologictolerance in small bowel transplantation of rat.关键词
微嵌合体/脾细胞输注/免疫耐受/小肠移植Key words
Chimerism/Splenocyte infusion/Immunologic tolerance/Small bowel transplantation分类
医药卫生引用本文复制引用
姜涛,姜峰奇,朱跃坤,朱安龙,朴大勋..脾细胞移植诱导大鼠小肠移植微嵌合体形成的实验研究[J].结直肠肛门外科,2012,18(2):125-127,3.基金项目
国家自然科学基金(30972878) （30972878）
