大豆科学2012，Vol.31Issue(1)：29-33,5.
引进大豆种质遗传转化适用基因型的筛选
Screening of the Optimal Acceptor Genotypes in Introduced Soybean Germplasm
摘要
Abstract
cryI gene was transformed into soybean cotyledon nodes of 6 foreign soybean germplasm by Agrobacterium-midiated method and screened the optimal acceptor genotype by comparing the callus induction, regeneration and etiolate rate. Then the suitable 6-BA concentration for germination and induction mediums of the optimal genotype was studied. The results indicated that the induction, differentiation and etiolation rate were obviously different between soybean genotypes. Among the 6 intro duced soybean germplasm,' Yuehe' was optimal for tissue culture and genetic transformation. The best hypocotyl-radicel ratio could be obtained when the concentration of 6-BA was 0. 1 mg-L"1. The optimal concentration of 6-BA for ' Yuehe' transfor mation was 0.1 and 1.7 mg ? L 'in germination medium and bud induction medium,respectively.关键词
大豆/引进种质/遗传转化/基因型/筛选Key words
Soybean/ Introduced germplasm/ Genetic transformation/ Genotype screening分类
农业科技引用本文复制引用
吴帅,王志坤,蓝岚,曾蕊,宋波,拓云,刘珊珊..引进大豆种质遗传转化适用基因型的筛选[J].大豆科学,2012,31(1):29-33,5.基金项目
国家自然科学基金资助项目(31071440) （31071440）
黑龙江省普通高等学校青年骨干支持计划项目(1155G12) （1155G12）
博士后研究人员落户黑龙江科研启动项目(2009HB009) （2009HB009）
转基因生物新品种培育重大专项资助项目(2011ZX08004-004-006-002). （2011ZX08004-004-006-002）
