大豆科学2012，Vol.31Issue(1)：34-37,4.
大豆幼胚培养直接成苗影响因素研究
Influence Factors of Plantlet Regeneration Directly from Cultured Young Embryos in Soybean
摘要
Abstract
In order to establish the method of plantlet regeneration directly from cultured young embryo of soybean, some influ encing factors including embryo age, pre-treatment methods, cultivation medium and soybean genotypes, were investigated re spectively. Germination rate of 15 d young embryo was 23% ,and that of 20 d or elder young embryo was more than 70% . Al though 20 d young embryo could germinate, the development of its bud and root was difficult. Among the 2 days pre-treatment of low temperature(4℃ ) ,high temperature(37℃ ) and GA buffer( 100 mmol·L-1 ) ,high-temperature promoted the plantlet rate(75% )of young embryo,while no significant effect were observed for other treatmeats. The tested cultivation medium con taining different external hormones had no obvious effect on plant regeneration. The plantlet rate of soybean genotypes varied with maturity groups,and that of extremely early-mature soybean were highest,with Ribenqing and Tainong 292 were 74% and 72% Respectively.关键词
大豆/幼胚培养/成苗Key words
Soybean/ Young embryo culture/ Plantlet分类
农业科技引用本文复制引用
何道一,张艳梅,张坤亮..大豆幼胚培养直接成苗影响因素研究[J].大豆科学,2012,31(1):34-37,4.基金项目
资源植物生物学安徽省重点实验室开放课题(KLPRB200910). （KLPRB200910）
