大豆科学2012，Vol.31Issue(1)：112-114,118,4.
大豆皂苷的微波辅助提取工艺及对酪氨酸酶活性影响的研究
Technology for Microwave-assistant Extraction of Soyasaponins from Bean Dregs and Its Effects on Tyosinase Activity
摘要
Abstract
Microwave-assistant extraction technology was used to obtain soyasaponins from bean dregs and the optimum tech nology parameters were determined by orthogonal experiment. Tyrosinase activity was determined by the dopachrome method u sing L-DOPA as the substrate and the amount of dopachrome in the reaction mixture was measured by spectrophotometer. The optimum technological parameters for soyasaponins extraction were obtained as follow; microwave extraction temperature at 40℃ , microwave extraction for 30 min, the solid-liquid ratio 1: 5 and extracted for 3 times. Under above conditions, the soyasa ponins content in bean dregs was 8. 11%. Soyasaponins had an appreciable antityrosinase activity and the inhibitory effect was enhenced with the concentration increasing, the value of half inhibitory concentration(IC50 ) was 222.7 μg·mL-1.关键词
大豆皂苷/微波辅助提取/酪氨酸酶活性Key words
Soyasaponin/ Microwave-assisted extraction/ Tyrosinase activity分类
轻工纺织引用本文复制引用
李雅晶,吴建阳,郑雅婷..大豆皂苷的微波辅助提取工艺及对酪氨酸酶活性影响的研究[J].大豆科学,2012,31(1):112-114,118,4.基金项目
浙江省新苗人才计划(2010R441001) （2010R441001）
浙江经贸职业技术学院创新项目(11CX03). （11CX03）
