大豆科学2012，Vol.31Issue(1)：127-130,4.
淡豆豉中多指标成分的含量测定方法研究
Determination Method of Multi-target Components in Semen Sojae Praeparatum
摘要
Abstract
To establish methods for the determination of Daidzein and Genistein in Semen Sojae Praeparatum by high perform ance liquid chromatography and total isoflavones by ultraviolet spectrophotometry. The established HPLC conditions were: an Agilent HC-C18 column(250×4. 6 mm,5.0 μm)was applied;lhe mobile phase was MeOH:0. L%Hac =50: 50;the tempera ture of column was 30℃ ;the flow rate was 1.0 mL·min-1; detective wavelength was set at 261 nm. Wavelength at 261 nm of ultraviolet spectrophotometry was adopted to determine the absorbance of samples solution. The results showed that the reten tion time of Daidzein and Genistein was 9. 79 and 14. 87 min.with the average recovery 98. 90% and 100. 53% ,respectively. The integral value of peak area has linear relationships with sample amount of Daidzein or Genistein( r >0. 9999) . Total isofla vones from 1.44 to 7.20 μg·mL-1 showed a linear relationship and the average recovery was 102.26%. The methods are sim ple and easy to evaluate the quality of Semen Sojae Praeparatum.关键词
淡豆豉/HPLC/UV/总异黄酮/大豆苷元/染料木素Key words
Semen Sojae Praeparatum/HPLC/UV/Total isoflavones/Daidzein/Genistein分类
轻工纺织引用本文复制引用
崔力剑,石素琴,刘敏彦,曹秀莲,牛丽颖..淡豆豉中多指标成分的含量测定方法研究[J].大豆科学,2012,31(1):127-130,4.基金项目
河北省自然科学基金资助项目(C2009001061). （C2009001061）
