大豆科学2012，Vol.31Issue(1)：131-134,4.
与纳豆激酶有亲和吸附特性的大豆蛋白性质研究
Properties of Soybean Proteins Having Affinity Adsorption with Nattokinase
摘要
Abstract
Using Sepharose-4B cross-linked with nattokinase as adsorbing medium, the target proteins were obtained from those of the soybean proteins by affinity chromatography. The molecular weights of target proteins were 30. 0 and 26. 0 kDa,respec tively, measured by degeneration electrophoresis technology. Surface plamon resonance technology was used to determine the affinity adsorption between nattokinase and target soy proteins and the results revealed the protein contains the structure that coupled to nattokinase and the target proteins might be the two subunit pairs of 11S(glycinin).关键词
纳豆激酶/亲和吸附/大豆蛋白/表面等离子共振技术Key words
Nattokinase/Affinity adsorption/Soy proteins/Surface plamon resonance technology分类
轻工纺织引用本文复制引用
李希生,史延茂,田智斌,董超..与纳豆激酶有亲和吸附特性的大豆蛋白性质研究[J].大豆科学,2012,31(1):131-134,4.基金项目
河北省科技支撑计划资助项目(11275508D). （11275508D）
