大豆科学2012，Vol.31Issue(2)：167-172,6.
根癌农杆菌介导的大豆子叶节转化体系的优化
Optimization of Agrobacterium-mediated Soybean Transformation using the Cotyledonary Node
摘要
Abstract
To optimize the system of Agrobacterium-mediated soybean[Glycine max (L. ) Merrill]transformation,effects of 13 cultivars and 2 selection agents were studied during the process of shoot regeneration and transformation. The results showed that the cultivar ' Shanning 14' induced the highest number of multiple shoot among tested 13 cultivars, and hygromycin B was better than glufosinate during transformant selection of cultivar ' Shanning 14'. The optimal transformant selection scheme was a gradient concentration of hygromycin B from 3 to 8 mg ?L-1. The integrations of UidA ( GUS) gene into the transformant genome were demonstrated by histological staining, PCR application and sequencing analysis. Transformation efficiency was 3. 2% based on the number of transformed events.关键词
大豆/农杆菌/子叶节/转化Key words
Soybean/Agrobacterium tumefaciens/Cotyledonary node/Transformation分类
农业科技引用本文复制引用
郝荣华,邵群,杨素欣,李继存,冯献忠,赵彦修..根癌农杆菌介导的大豆子叶节转化体系的优化[J].大豆科学,2012,31(2):167-172,6.基金项目
山东省自然科学杰出青年基金资助项目(JQ200909) （JQ200909）
国家自然科学基金资助项目(31171571). （31171571）
