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适于子叶节和胚尖再生体系的大豆基因型筛选

郑丽红 季静 王罡 关春峰 王萍 王婉婉

大豆科学2012,Vol.31Issue(2):213-216,4.
大豆科学2012,Vol.31Issue(2):213-216,4.

适于子叶节和胚尖再生体系的大豆基因型筛选

Selection of Suitable Soybean Genotype Based on Cotyledon Node and Embryonic Tip Regeneration Systems

郑丽红 1季静 2王罡 2关春峰 2王萍 2王婉婉3

作者信息

  • 1. 天津大学化工学院,天津300072
  • 2. 天津大学遗传工程研究所,天津300072
  • 3. 淮海工学院海洋学院,江苏连云港222005
  • 折叠

摘要

Abstract

Cotyledon nodes and embryonic tips of soybean were used as explants to investigate the induction rate of adventitious shoots, elongation rate of adventitious shoots and the number of adventitious shoots. The results showed that genotype could affect regeneration frequency obviously in the selected regeneration system. For Hefeng35 and Hefeng25, both induction rates and elongation rates in cotyledon node regeneration system were higher than those in embryonic tip regeneration system,while the induction rates and elongation rates of Heinong37 were opposite. Different regeneration system should be applied when different soybean genotypes were used. Hefeng35, Hefeng25 and Suinongl4 were suitable for cotyledon node regeneration system, Hei-nong37 was more suitable for the embryonic tip regeneration system. Furthermore,Jiyu91 and Beijingxiaolidou were suitable for both regeneration systems.

关键词

大豆/基因型/子叶节/胚尖/再生体系

Key words

Soybean/Genotype/Cotyledon node/Embryonic tip/Regeneration system

分类

农业科技

引用本文复制引用

郑丽红,季静,王罡,关春峰,王萍,王婉婉..适于子叶节和胚尖再生体系的大豆基因型筛选[J].大豆科学,2012,31(2):213-216,4.

基金项目

国家转基因生物新品种培育重大专项(2008ZX08004-001,2009ZX08010-013B). (2008ZX08004-001,2009ZX08010-013B)

大豆科学

OA北大核心CSCDCSTPCD

1000-9841

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