大豆科学2012,Vol.31Issue(2):213-216,4.
适于子叶节和胚尖再生体系的大豆基因型筛选
Selection of Suitable Soybean Genotype Based on Cotyledon Node and Embryonic Tip Regeneration Systems
摘要
Abstract
Cotyledon nodes and embryonic tips of soybean were used as explants to investigate the induction rate of adventitious shoots, elongation rate of adventitious shoots and the number of adventitious shoots. The results showed that genotype could affect regeneration frequency obviously in the selected regeneration system. For Hefeng35 and Hefeng25, both induction rates and elongation rates in cotyledon node regeneration system were higher than those in embryonic tip regeneration system,while the induction rates and elongation rates of Heinong37 were opposite. Different regeneration system should be applied when different soybean genotypes were used. Hefeng35, Hefeng25 and Suinongl4 were suitable for cotyledon node regeneration system, Hei-nong37 was more suitable for the embryonic tip regeneration system. Furthermore,Jiyu91 and Beijingxiaolidou were suitable for both regeneration systems.关键词
大豆/基因型/子叶节/胚尖/再生体系Key words
Soybean/Genotype/Cotyledon node/Embryonic tip/Regeneration system分类
农业科技引用本文复制引用
郑丽红,季静,王罡,关春峰,王萍,王婉婉..适于子叶节和胚尖再生体系的大豆基因型筛选[J].大豆科学,2012,31(2):213-216,4.基金项目
国家转基因生物新品种培育重大专项(2008ZX08004-001,2009ZX08010-013B). (2008ZX08004-001,2009ZX08010-013B)