大豆科学2012，Vol.31Issue(2)：247-251,5.
外源谷胱甘肽对大豆种子萌发过程中铜毒害的缓解效应
Alleviation Effects of Exogenous Glutathione on Copper Toxicity during Soybean Seeds Germination
摘要
Abstract
The alleviation effects of exogenous GSH on copper toxicity during soybean seeds germination was studied in this paper. The soybean seeds were soaked in solution with different copper concentrations of 0,0.5,1.0,2.0,4.0 mmol ?L-1, supplied with 0.16 and 0.32 mmol-L"1 GSH,and then the seeds germination rate,relative permeability,praline content and activity of a-amylase were investigated. The results showed that the copper concentration of 1.0 mmol-L-1 or above evidently decreased the activity index and radicle length,but the relative electrolyte permeability and proline content were enhanced remarkably; 2.0 mmol'L"1 or above copper concentration notably reduced the germination index and activity of a-amylase; 4.0 mmol-L"1 copper observably restrained the germination rate. Under 1. 0 mmol 'L-1 or above copper stress,0. 16 and 0.32 mmol稬-1 GSH markedly improved the activity of a-amylase and radicle length,but reduced proline content. Under 2.0 mmol'L"1 or above copper stress,0.16 and 0.32 mmol'L'1 GSH evidently decreased the relative electrolyte permeability. Under 4.0 mmol ?L-1 copper stress ,0.32 mmol ?L -1 GSH remarkably enhanced the germination rate, germination and activity index. These results indicated that 0.16 and 0.32 mmol ?L-1 GSH improved the ability of soybean seeds germination via enhancing the activity of a-amylase,meanwhile,alleviated copper toxicity on soybean seeds in a certain extent to maintain the integrity of cell membrane.关键词
铜毒害/GSH/大豆/α-淀粉酶/相对电导率/脯氨酸Key words
Copper stress/Glutathione/Soybean/a-amylase/Relative permeability/Proline分类
农业科技引用本文复制引用
陈玉胜..外源谷胱甘肽对大豆种子萌发过程中铜毒害的缓解效应[J].大豆科学,2012,31(2):247-251,5.基金项目
江苏省科技厅资助项目(BE2009375). （BE2009375）
