动物学研究2012，Vol.33Issue(2)：211-217,7.DOI:10.3724/SP.J.1141.2012.02211
IFN-γ、IL-1α、NGF-β和TNF-α在皖西白鹅小脑皮质发育中的表达
Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose
摘要
Abstract
The strep avidin-biotin-peroxidase complex (SABC) immunohistochemical methods were applied to investigate the localization and semi-quantitative distribution of IFN-γ, IL-lα, NGF-β and TNF-a-immunoreactive cells in the cerebellar cortex of Western Anhui white goose at embryonic day 13, 19, 24, 28 (E13, E19, E24, E28) and postnatal day 7, 15 (P7, P15). The possible roles of IFN-γ, IL-lα NGF-β and TNF-a in the development of cerebellar cortex were discussed. The results indicated that in the external granular layer, there were IFN-γ and TNF-α positive cells at E13, E19, E24, E28, P7, IL-lα positive cells at E13, E19, E24, E28 and NGF-β positive cells at E13, E19 , E24. The expression levels of these four cytokines all reached peaks at El 9 of the six tested periods in this study. In the Purkinje cell layer, there were IFN-γ, IL-lα and TNF-α positive cells at E13, E19, E24, E28, P7, P15 and NGF-β positive cells at E13, E19, E24, E28, P7. In the internal granular layer, there were IFN-y positive cells at E13, E19, E24, E28, P7, P15, IL-la and TNF-α positive cells at E13, E19, E24, E28, P7 and NGF-β positive cells at E13, E19, E24, E28. These results showed that E19 might be the "critical stage" in the cerebellar cortex development of Western Anhui white goose. IFN-γ, IL-lα and TNF-α might be synthesized by cerebellar cortex itself, and NGF-β could be transported from regions which project to Purkinje cells. IFN-γ may interfer the transfer of granular cells, and NGF-β may have neurotrophic functions that are beneficial to the growth and development of Purkinje cells.关键词
皖西白鹅/小脑皮质/发育/细胞因子/免疫组织化学Key words
Western Anhui white goose/ Cerebellar cortex/ Development/ Cytokines/ Immunohistochemistry分类
生物科学引用本文复制引用
宋海燕,刘再群,郑磊..IFN-γ、IL-1α、NGF-β和TNF-α在皖西白鹅小脑皮质发育中的表达[J].动物学研究,2012,33(2):211-217,7.基金项目
安徽师范大学博士科研启动基金(160-750932),生科院联合开放课题经费 （160-750932）
“安徽重要生物资源保护与利用研究”安徽省重点实验室科研基金 （）
