动物学研究2012，Vol.33Issue(2)：225-230,6.DOI:10.3724/SP.J.1141.2012.02225
一种新的可用于干细胞移植的新西兰白兔青光眼视神经损伤模型
Glaucoma model for stem cell transplantation research in New Zealand white rabbits
摘要
Abstract
Glaucoma is a typical irreversible blind neurodegenerative disease for which there is no effective treatment for halting visual deterioration. The recent development of neural stem cells studies sheds light on a potential resolution for this disease. As a result, an appropriate glaucoma modeling method for stem cell transplantation study is needed. In the present study, Dexamethasone was injected unilaterally into the conjunctiva of New Zealand rabbit at the dose of 2.5 mg (5 mg/mL), three times a week. After eight weeks, the eye ground photography showed that the optic nerve head of the treated eye was expanded, and the blood vessel was geniculate compared to the control eye, while the ocular media remained transparent. The hematoxylin-eosin (HE) stain of the retinal nerve fiber layer (RNFL) sections showed optic neuron death in the treated eye. The Heidelberg Retina Tomography (HRT) results showed optic disk morphological changes consistent with the pathophysiology of glaucoma in the treated eye, including a decrease in the rim area (1.10±0.88) mm2 and mean RNFL thickness (0.44±0.31) mm, and an increase in the cup/disk ratio 0.17±0.13. Then neural stem cells were injected into the vitreous body of the treated eye. After five months, surviving transplanted cells were observed. These results suggest a simple and reproducible chronic glaucoma model, which is appropriate for neural stem cell transplant research, has been successfully developed.关键词
青光眼/动物模型/地塞米松/干细胞移植Key words
Glaucoma/ Animal model/ Dexamethasone/ Stem cells transplantation分类
生物科学引用本文复制引用
郭立云,魏景宽,杨上川,王正波..一种新的可用于干细胞移植的新西兰白兔青光眼视神经损伤模型[J].动物学研究,2012,33(2):225-230,6.基金项目
“973”项目(947703) （947703）
国家自然基金项目(31070963,30670669) （31070963,30670669）
