动物营养学报2012，Vol.24Issue(3)：479-486,8.DOI:10.3969/j.issn.1006-267x.2012.03.014
壳寡糖对虹鳟生长性能、血清生化指标及非特异性免疫功能的影响
Effects of Chito-Oligosaccharides on Growth Performance,Serum Biochemical Indices and Non-Specific Immunity Function of Rainbow Trout (Oncorhynchus mykiss)
摘要
Abstract
This experiment was conducted to evaluate the effects of chito-oligosaccharides on growth performance, serum biochemical indices and non-specific immunity function of rainbow trout ( Oncorhynchus mykiss). A total of 480 fish with the body weight about 60 g were randomly allotted to 4 groups with 4 replicates each and each replicate contained 30 fish. The fish in the 4 groups were fed a basal diet supplemented with 0, 100, 200 or 400 mg/kg chito-oligosaccharides, respectively. The experiment lasted for 49 days. The results showed as follows: 1) compared with the control group, the specific growth rate in the 100, 200 and 400 mg/kg chito-oligosaccharides groups was increased by 5.60% (P < 0. 05), 15.89% (P <0. 05) and 18. 69% (P < 0.05) , the weight gain rate was increased by 7.53% (P <0.05) , 21.21% (P<0.05) and26.02% (P< 0.05), and the protein efficiency rate was increased by 0.60% (P >0.05), 7.02% (P >0.05) and 9.79% (P<0.05), while the feed conversion rate was decreased by 0.60% (P>0.05), 6. 55% (P>0.05) and 8. 93% (P<0.05), respectively. 2) Dietary supplementation of chito-oligosaccharides improved the contents of total protein, albumin, globulin in serum (P >0.05), while decreased the contents of triglyceride, cholesterol and glucose, and activities of glutamic pyruvic transaminase and glutamic oxaloacetic transaminase in serum (P>0.05). 3) Dietary supplementation of 200 and 400 mg/kg chito-oligosaccharides significantly improved the phagocytic percentage (P < 0.05 ) and phagocytic index of leucocyte (P < 0. 05), serum bactericidal percentage (P < 0. 01) and lysozyme activity in serum, liver and gill (P<0.05). 4) No significant differences were found in the contents of moisture, crude protein, crude lipid and ash of whole body among all groups (P >0. 05). It is concluded that dietary supplementation of chito-oligosaccharides can improve the growth performance and non-specific immunity function of rainbow trout, and the supplemental level of chito-oligosaccharides in diets is suggested to be 200 mg/kg.关键词
虹鳟/壳寡糖/生长性能/血清生化指标/非特异性免疫功能Key words
rainbow trout/ chito-oligosaccharides/ growth performance/ serum biochemical indices/ non-specific immunity function分类
农业科技引用本文复制引用
刘含亮,孙敏敏,王红卫,万文菊,王纪亭..壳寡糖对虹鳟生长性能、血清生化指标及非特异性免疫功能的影响[J].动物营养学报,2012,24(3):479-486,8.基金项目
山东省中青年科学家科研奖励基金(2006BS06018) （2006BS06018）
