南方医科大学学报2012，Vol.32Issue(4)：443-448,6.DOI:10.3969/j.issn.1673-4254.2012.04.002
人胎端脑额叶代谢型谷氨酸受体5的发育性表达及细胞定位
Developmental expression and cellular distribution of metabotropic glutamate receptor 5 in the frontal cortex of human fetus
摘要
Abstract
Objective To investigate the expression of metabotropic glutamate receptor 5 (mGluR5) and its cellular distribution in the frontal cortex,ventricular zone (VZ) and subventricular zone (SVZ) in human fetuses.Methods According to the gestational age,the collected fetuses were divided into 4 groups,namely 9-11 weeks,14-16 weeks,22-24 weeks and 32-36 weeks.Brain tissue blocks including the frontal lobe or VZ/SVZ were prepared into slices,and the expression pattern and cellular distribution of mGIuR5 in the frontal cortex and VZ/SVZ were observed by imrnunohistochemistry or immunofluorescence.Results mGluR5 immunoreactivity was present in the cell membrane in the frontal cortex,VZ and SVZ from the 9th to 36th weeks and the immunoreactivity in the marginal zone (MZ) and cortical plate (CP) was markedly stronger than that in VZ and SVZ.The cells expressing mGIuR5 included neural stem/progenitor cells in the VZ and SVZ,immature neurons in the VZ and MZ,and numerous mature neurons in the CP.Conclusion mGluR5 is expressed by a variety of cells such as neural stem cells in the frontal cortex,VZ and SVZ in human fetus,suggesting a role of mGluR5 in the development of human cerebral cortex.关键词
代谢型谷氨酸受体5/神经干/祖细胞/大脑皮质/人类胚胎Key words
metabotropic glutamate receptor 5/neural stem/progenitor cells/cerebral cortex/human fetuses分类
医药卫生引用本文复制引用
解武玲,杨蓬勃,张军峰,肖新莉,靳辉,石秦东,徐曦,刘勇..人胎端脑额叶代谢型谷氨酸受体5的发育性表达及细胞定位[J].南方医科大学学报,2012,32(4):443-448,6.基金项目
国家自然科学基金(81070998) （81070998）
西安交通大学基本科研业务费(xjj201122) （xjj201122）
