南方医科大学学报2012，Vol.32Issue(4)：482-486,5.DOI:10.3969/j.issn.1673-4254.2012.04.009
Wistar大鼠实验性自身免疫性脑脊髓炎轴索损害的病理学分析
Pathological findings of axonal injury in a rat model of experimental allergic encephalomyelitis
摘要
Abstract
Objective To observe the pathological changes of axonal injury in a rat model of experimental allergic encephalomyelitis (EAE).Methods With HE,luxol fast blue and Bielschowsky staining,the expression of APP,MBP,SMI-32 and MBP in the brain and spinal cord of EAE rats using double-labeling indirect immunofluorescence.Results Extensive cuffing lesions of inflammatory cell infiltrations were found in the brain and spinal cord of the rats,accompanied by multiple lesions of demyelination,axonal disarrangement with vesicular loss.SMI-32 staining identified numerous nonphosphorylated neurofilament,indicating the presence of axonal injury.Axonal oval bodies formed by APP accumulation were found in the white matters of the spinal cord 14 days after EAE,suggesting that neuraxial damage occurred in the early stage of EAE which was not synchronous with myelin loss.Conclusion Different levels of inflammation occur in different stages of EAE,and inflammatory cell infiltration is the most obvious at the peak of EAE.Axonal injury occurs in the early stage of EAE and progresses over the entire disease course.关键词
多发性硬化/实验性脑脊髓炎/轴索损害/淀粉样前体蛋白/髓鞘脱失Key words
multiple sclerosis/experimental allergic encephalomyelitis/axon injury/amyloid precursor protein/demyelination分类
医药卫生引用本文复制引用
王圆圆,吕田明,刘晓加,方敏,梁彦珊..Wistar大鼠实验性自身免疫性脑脊髓炎轴索损害的病理学分析[J].南方医科大学学报,2012,32(4):482-486,5.基金项目
国家自然科学基金(61072033) （61072033）
