南方医科大学学报2012，Vol.32Issue(4)：531-533,564,4.DOI:10.3969/j.issn.1673-4254.2012.04.020
2型糖尿病患者与健康个体间肠道双歧杆菌的差异
Differences in fecal Bifidobacterium species between patients with type 2 diabetes and healthy individuals
摘要
Abstract
Objective To determine the changes in fecal Bifidobacterium species in patients with type 2 diabetes in comparison with healthy individuals.Methods The bacterial DNA were extracted from the fecal samples from 50 type 2 diabetic patients and 30 healthy individuals.Real-time quantitative PCR was employed to determine the copy numbers of the bacteria in the fecal samples using 16S rRNA-targeted genus- and species-specific PCR primers for a selected group of fecal Bifidobacterium species including total Bifidobacterium,B.longum,B.breve,B.adolescentis,and B.infantis.Results The diabetic group had significantly lower copy numbers of total Bifidobacterium and B.adolescentis compared to the healthy individuals (P<0.05).Conclusion Type 2 diabetic patients have a lowered number of Bifidobacterium species in the gut microflora.关键词
肠道菌群/双歧杆菌/2型糖尿病/实时荧光定量PCRKey words
intestinal flora/Bifidobacterium species/type 2 diabetes/real-time quantitative polymerase chain reaction分类
医药卫生引用本文复制引用
许小津,惠宏襄,蔡德鸿..2型糖尿病患者与健康个体间肠道双歧杆菌的差异[J].南方医科大学学报,2012,32(4):531-533,564,4.基金项目
国家973重点基础研究发展计划(2011CB504006) （2011CB504006）
