南方医科大学学报2012，Vol.32Issue(4)：569-572,4.DOI:10.3969/j.issn.1673-4254.2012.04.029
芥菜籽对小鼠变应性接触性皮炎的抑制作用
Effects of mustard seed on 2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice
摘要
Abstract
Objective To investigate the therapeutic effect of mustard seed on allergic contact dermatitis (ACT)) in mice and explore the mechanism.Methods Eighteen BALB/c mice were randomly divided into normal control group,model group and mustard seed group.The mice in the normal control group and model group were fed with normal chow,and those in mustard seed group were given 5% mustard seed mixed in the chow.Three weeks later,ACD was induced on the ear using 2,4-dinitrofluorobenzene.After 24 h,the swelling of the ear was examined,and the rats were sacrificed to collect the ear tissue ears and blood for histopathological and immunohistochemical examinations,RT-PCR and enzyme-linked immunosorbent assay.Results In mice with ACD,feeding with mustard seeds significantly lessened the ear swelling,improved the tissue histopathology,lowered the number of infiltrating Langerhans cells,and reduced the expressions of IL-1β,TNF-α and IL-6 mRNA in the ear,but did not cause significant changes in serum levels of IL-4,IFN-γ and IL-17.Conclusion Mustard seed inhibits ACD in mice possibly by suppressing the expressions of IL-lβ TNF-α and IL-6 mRNA and inhibiting Langerhans cell migration in the epidermis.关键词
芥菜籽/二硝基氟苯/变应性接触性皮炎/朗格汉斯细胞Key words
mustard seed/2,4-dinitrofluorobenzene/allergic contact dermatitis/langerhans cells分类
医药卫生引用本文复制引用
汶春苗,杨闰平,胡坚,焦泽龙,杨宇光,杨剑凌,李恒进,赵华..芥菜籽对小鼠变应性接触性皮炎的抑制作用[J].南方医科大学学报,2012,32(4):569-572,4.基金项目
解放军总医院苗圃基金(01020604) （01020604）
