南方医科大学学报2012，Vol.32Issue(4)：575-579,5.DOI:10.3969/j.issn.1673-4254.2012.04.031
1,25二羟基维生素D3联合5-氟尿嘧啶对人食管癌Eca-109细胞移植瘤的影响
Effect of 1,25-dihydroxyvitamin D3 combined with 5-fluorouracil on IGFBP-3 expression in human esophageal carcinoma 109 cell xenograft in nude mice
摘要
Abstract
Objective To investigate the effect of 1,25-dihydroxyvitamin D3 and 5-eluorouracil,either alone or in combination,on the expression of IGFBP-3 in human esophageal carcinoma 109 cell xenograft in nude mice.Methods In vitro cultured esophageal carcinoma Eca-109 cells were inoculated subcutaneously in BALB/c mice.The tumor-bearing mice were randomly devided into control group (A),1,25-dihydroxyvitamin D3 group (B),5-fluorouracil group (C),and 1,25-dihydroxyvitamin D3 plus 5-fluorouracil group (D).1,25-dihydroxyvitamin D3 and 5-fluorouracil were administered at the doses of 2.5 μg/kg and 25 mg/kg via intraperitoneal injections,respectively,and the mice in the control group received salinel injection only.The tumor growth was observed and the expression of IGFBP-3 in the tumor xenograft was detected using immunohistochemistry.An automatic biochemistry analyzer was used to determine serum calcium levels,and Von Kossa staining was utilized for observation of calcium deposition in the kidneys.Results Compared with that in group A,the xenograft in groups B,C,and D all showed a lowered growth rate with a smaller tumor volume,and presented with stronger IGFBP-3 positivity and significantly higher levels of IGFBP-3 protein expression (P<0.05).In group D,the protein expression of IGFBP-3 was significantly increased compared with that in groups B and C (P<0.05).Compared with that in group A,serum calcium level was slightly increased in groups B,C,and D,,but no obvious calcium deposition was found in the kidney tissue sections.Conclusion Both 1,25-dihydroxyvitamin D5 and 5-fluorouracil can inhibit the growth of the tumor xenograft in nude mice,and their combination is more effective.This effect is probably associated with increased protein expression of IGFBP-3 in the xenograft tumor.No calcium deposition occurs in the kidney tissue of the tumor-beaimg mice.关键词
食管癌/1,25二羟基维生素D3/5-氟尿嘧啶/胰岛素生长因子结合蛋白-3/钙沉积Key words
esophageal carcinoma/1,25-dihydroxyvitamin D3/5-fluorouracil/IGFBP-3/calcium deposition分类
医药卫生引用本文复制引用
李钢,蒋迎九,吴庆琛,李强,余敏,汤为学..1,25二羟基维生素D3联合5-氟尿嘧啶对人食管癌Eca-109细胞移植瘤的影响[J].南方医科大学学报,2012,32(4):575-579,5.基金项目
重庆市渝中区科技计划项目(2010126) （2010126）
