福建农林大学学报（自然科学版）2012，Vol.41Issue(1)：13-17,5.
TC/IC-RT-PCR检测黄瓜绿斑驳花叶病毒
Detection of cucumber green mottle mosaic virus by TC/IC-RT-PCR
摘要
Abstract
Specific primers were designed based on the conserved region of coat protein ( CP) gene of Cucumber green mottle mosaic virus (CGMMV). Tube capture RT-PCR (TC-RT-PCR) and immunocapture RT-PCR (IC-RT-PCR) were employed to detect CGMMV. The results showed that two assay technologies had high specificity. Sensitivity provided by TC-RT-PCR and IC-RT-PCR was 10 and 100 times higher than that of DAS-ELISA, respectively. The application effects of two technologies were confirmed by means of detecting CGMMV in imported cucumber samples. TC-RT-PCR and IC-RT-PCR developed in this study can be effectively used for the rapid detection of CGMMV.关键词
黄瓜绿斑驳花叶病毒/试管捕捉RT-PCR/免疫捕捉RT-PCRKey words
Cucumber green mottle mosaic virus/ tube capture RT-PCR/ immunocapture RT-PCR分类
农业科技引用本文复制引用
沈建国,林双庆,蔡伟,李海明,王念武,翁瑞泉,黄可辉..TC/IC-RT-PCR检测黄瓜绿斑驳花叶病毒[J].福建农林大学学报（自然科学版）,2012,41(1):13-17,5.基金项目
福建省自然科学基金项目(2009J01046) （2009J01046）
福建省科技计划重点项目(2009N0001) （2009N0001）
福建出入境检验检疫局科技项目(FK2011-07、FK2008-05、FK2006-19). （FK2011-07、FK2008-05、FK2006-19）
