福建农林大学学报（自然科学版）2012，Vol.41Issue(1)：73-77,5.
7株新城疫病毒V基因遗传变异与病毒的基因分型
Genetic variation of V gene from seven isolates of Newcastle disease viruses and its relationship with virus genotype
摘要
Abstract
V protein is one of the accessory proteins of Newcastle disease viruses produced during the process of genome transcription by RNA editing. It is an interferon antagonist, which assists viruses to avoid host immune response, and may affect the pathoge-nicity and host range restriction of virus. In this study, nucleotide homology and phylogenetic analysis of V genes of seven isolates were performed. Comparison of V gene sequences showed nucleotide identity of 81.7% -99.7% and deduced amino acid identities of 75.8% -99.6% among the seven isolates. Phylogenetic analysis revealed that the isolates were divided into 3 different clusters, and strains with similar virulence were clustered together. The topology of phylogenetic tree based on the V genes was consistent with the tree based on fusion (F) genes of the viruses, which was used for genotype analysis. The results indicated that the nucleotide acids mutation rate of V genes resembled that of F genes, and V genes would also be suitable for genotype analysis of Newcastle disease viruses.关键词
V基因/遗传变异/新城疫病毒Key words
V gene/ genetic variation/ avian Newcastle disease viruses分类
农业科技引用本文复制引用
傅光华,林建生,黄瑜,程龙飞,施少华,刘友生,彭春香,陈红梅,万春和,林芳..7株新城疫病毒V基因遗传变异与病毒的基因分型[J].福建农林大学学报（自然科学版）,2012,41(1):73-77,5.基金项目
国家自然科学基金资助项目(39072203) （39072203）
现代农业产业技术体系建设专项资金资助项目(CARS-43) （CARS-43）
福建省自然科学基金资助项目(2009J01097) （2009J01097）
福建省农业科学院青年科技人才创新基金资助项目(2008QB-4) （2008QB-4）
福建省财政专项——福建省农业科学院科技创新团队建设基金资助项目(STIF-Y02). （STIF-Y02）
