福建农林大学学报（自然科学版）2012，Vol.41Issue(2)：153-158,6.
干扰隐花色素Cry1a与Cry1b基因的表达对水稻若干农艺性状的影响
Effects of interfering the expression of cryptochrome Cry1a/Cry1b genes on several rice agronomic traits
摘要
Abstract
According to the published rice Cry I a and Crylb gene sequences, we designed the related primers to get part of the gene fragments by PCR and constructed an ihpRNA plant expression vector named pSC1301-347-CrrlaCrrl6, then introduced it into rice by Agrobacterium-medisteA genetic transformation so as to cause the functional loss of the two genes at the same time. The effects of rice Cryla and Crylb on important agronomic traits were preliminarily analyzed based on the performance of the transformants. The results indicated that the transgenic plants retarded flowering over 16 d; the plant height increased significantly with the grain shape evidently becoming longer while the other traits observed did not change apparently.关键词
水稻/隐花色素/Cry1a/Cry1b基因/ihpRNA/农艺性状Key words
rice/ cryptochrome/ Cryla/Crylb gene/ ihpRNA/ agronomic traits分类
农业科技引用本文复制引用
李毓,庄伟建,庄春红,王乃元,戴飞..干扰隐花色素Cry1a与Cry1b基因的表达对水稻若干农艺性状的影响[J].福建农林大学学报（自然科学版）,2012,41(2):153-158,6.基金项目
福建省科技计划重点项目(2008101021117) （2008101021117）
国家高技术研究发展计划(2007AA10Z181)资助项目. （2007AA10Z181）
