福建农林大学学报（自然科学版）2012，Vol.41Issue(2)：159-162,4.
苏云金芽孢杆菌外孢囊蛋白基因敲除载体的构建
Construction of the exosporium protein gene knockout vector in Bacillus thuringiensis
摘要
Abstract
Bacillus thuringiensis subsp. Kurstaki isolate 8010 was selected to obtain the upstream and downstream of exosporium protein gene by PCR technology. The pDG780 vector ( containing the kanamycin resistance gene) was used to construct a recombination plasmid pRN5101UKD which contained the upstream sequence of exosporium protein gene, the kanamycin resistance gene and the downstream sequence of exosporium protein gene by PCR and RE treatments. It was provided for further study on gene knockout.关键词
苏云金芽孢杆菌/外孢囊蛋白基因/基因敲除载体Key words
Bacillus thuringiensis/ exosporium protein gene/ gene knockout vector分类
生物科学引用本文复制引用
李今煜,潘智雄,黄天培..苏云金芽孢杆菌外孢囊蛋白基因敲除载体的构建[J].福建农林大学学报（自然科学版）,2012,41(2):159-162,4.基金项目
福建省高校服务海西建设重点项目(0b08b005、0685B05K) （0b08b005、0685B05K）
福建省教育厅科技项目(JK2009012). （JK2009012）
