福建农业科技Issue(3)：111-115,5.
蝴蝶兰丛生芽快速繁殖体系的建立
Establishment of technique system of rapid propagation through clustered buds pathway of Phalaenopsis
摘要
Abstract
Phalaenopsis cultivar Dtps.Tailin Red Angel ＂V31＂ was used as tissue culture material,investigations on the effect of disinfection time,basic medium,exogenous hormone on the induction of pedicel axillary bud,and effect of exogenous hormone on the propagation,rooting ability and vigorous condition of clustered buds were carried out.The results showed that when disinfected with 0.1% HgCl2 for 8 min,the induction rate of pedicel axillary bud were highest（85.19%）.The induction rate of pedicel axillary bud were significantly higher on 1/2MS medium than MS medium.When the buds inoculated into 1/2MS＋NAA 0.5mg/L＋coconut juice 100ml/L＋sucrose 30g/L＋agar 7g/L with 5.0 mg/L 6-BA,the multiplication coefficient was highest（5.53）;Adding coconut juice 100ml/L in 1/2MS＋5.0 mg/L 6-BA＋NAA 0.5mg/L＋sucrose 30g/L＋agar 7g/L,the multiplication coefficient was significantly higher than mashed potatoes and banana slurry.When the plantlets without roots inoculated into 1/2MS ＋mashed potatoes 80g/L＋sucrose 20g/L＋ agar 7g/L with 0.5 mg/L NAA,the numbers of rooting and leaf were significantly higher than other treatments,were 4.39 and 3.03;Adding mashed potatoes 80g/L in 1/2MS＋NAA 0.5mg/L＋sucrose 20g/L＋agar 7g/L,the numbers of rooting and leaf were significantly higher than coconut juice and banana slurry.关键词
蝴蝶兰/快繁/增殖/生根壮苗Key words
Phalaenopsis/rapid propagation/multiplication/rooting and vigorous condition of plantlets分类
农业科技引用本文复制引用
马晓娟,陈瑶瑶,庄卫东,刘靓,黄枝英..蝴蝶兰丛生芽快速繁殖体系的建立[J].福建农业科技,2012,(3):111-115,5.基金项目
福建省泉州市农业计划重点项目 （）
