福建农业学报2012，Vol.27Issue(1)：37-42,6.
香蕉种质资源亲缘关系的ISSR分析
Genetic Relationship among Banana (Musa spp.) Germplasms Revealed by ISSR Analysis
摘要
Abstract
Genetic diversity and relationship among 35 banana germplasms involving 3 genotypes (I. e. , AAA, AA and ABB) were studied by using the Inter-Simple Sequence Repeat (ISSR) method. As a result, 6 ISSR primers were selected, 38 bands amplified between 200 bp and 2, 000 bp, 27 bands detected for polymorphic DNA, and the percentage of polymorphic DNA 71. 05% of polymorphic DNA were found. The genetic distances among the banana cultivars were between 4. 30% and 33. 33%. A cluster analysis was performed applying the unweighted pair group method on arithmetic averages. The banana germplasms were grouped into 6 clusters based on the genetic distance of 25%. Except for Gongjiao (AA) and Jinfenl (ABB), which were grouped into Cluster III and IV, respectively, the remaining 33 germplasms were classified into 4 clusters, in which Cluster I contained 22 germplasms from different localities and genetypes (AAA and ABB). All germplasms with genotypes AAA and ABB could not be divided into 2 clusters. There appeared no close genetic relationship among some banana germplasms with same genotypes. The germplasms could be discriminated by the 6 selected primers, and ISSR was considered as an effective method for studying genetic relationship among different banana germplasms resources.关键词
香蕉/亲缘关系/ISSR/聚类分析Key words
Banana (Musa spp)/ genetic relationship/ ISSR/ cluster analysis分类
农业科技引用本文复制引用
姚锦爱,蔡鸿娇,石妞妞,甘林,杨秀娟,陈福如..香蕉种质资源亲缘关系的ISSR分析[J].福建农业学报,2012,27(1):37-42,6.基金项目
福建省自然科学基金项目(2011J01114) （2011J01114）
福建省发改委高新技术项目(2011-352) （2011-352）
农业科技成果转化资金项目(2009GB2C400174) （2009GB2C400174）
福建省科技创新平台建设项目(2009N2005) （2009N2005）
