福建农业学报2012，Vol.27Issue(3)：283-286,4.
苏云金芽胞杆菌LSZ9408菌株基因型的鉴定
Identification of Cry-type Gene of Bacillus thuringiensis Strain LSZ 9408
摘要
Abstract
PCR and SDS-PAGE were used to identify the cry-type gene of Bacillus thuringiensis (Bt) strain LSZ 9408. PCR analysis showed that Bt LSZ 9408 strain contained cry1 and cry2 genes. Five pairs of general primers-Un1, Un2, Un3, Un4 and Un7/8 were used to amplify PCR, and then the amplification results were detected by SDS-PAGE. Primes Unl and Un2 formed about 270 bp and 700 bp DNA fragments on agarose gel electrophoresis, while none PCR products was found with the other three pairs of primers. The results of SDS-PAGE also indicated that Bt LSZ 9408 possessed cry1 and cry2 genes, which coded 130kD and 65kD ICPs.关键词
LSZ 9408菌株/杀虫晶体蛋白基因/聚合酶链式反应/聚丙烯酰胺凝胶电泳Key words
Bt LSZ 9408 strain/ cry-type gene/ PCR analysis/ SDS-PAGE分类
农业科技引用本文复制引用
林营志,朱育菁,刘波..苏云金芽胞杆菌LSZ9408菌株基因型的鉴定[J].福建农业学报,2012,27(3):283-286,4.基金项目
国家"948"项目子专题(2011-G25) （2011-G25）
福建省科技计划项目——省属公益类科研院所基本科研专项(2009R10037-1) （2009R10037-1）
福建省杰出青年科学基金项目(2009J06010) （2009J06010）
