福建水产2012，Vol.34Issue(2)：134-140,7.
罗非鱼碎肉酶法制备蛋白胨的工艺研究
Study on the preparation of peptone from remnant muscles of Tilapia Surimi by different enzymes
摘要
Abstract
In this paper,orthogonal test method was used to study the preparation of peptone from remnant muscles of Tilapia surimi hydrolyzed by papain,AS.1398 neutral protease and protamex separately.Results showed that optimization hydrolysis conditions of papain were pH value with 6.5,temperature with 65℃,time of hydrolysis with 4h,and enzyme concentration with 1250U/g,the peptone yield was 12.63%.Optimization hydrolysis conditions of AS.1398 neutral protease were pH value with 7.5,temperature with 55℃,time of hydrolysis with 4h and enzyme concentration with 750U/g,the peptone yield was 13.25%.Optimization hydrolysis conditions of protamex were pH value with 7.0,temperature with 50℃,time of hydrolysis with 4.5h,and enzyme concentration with 850U/g,the peptone yield was 11.43%.关键词
罗非鱼碎肉/酶解/蛋白胨Key words
Tilapia surimi/Hydrolysis/Peptone分类
农业科技引用本文复制引用
朱思咪,张萍,陈丽娇,程艳..罗非鱼碎肉酶法制备蛋白胨的工艺研究[J].福建水产,2012,34(2):134-140,7.基金项目
福州市科技重点项目2009-N-51 （）
