分析化学2012，Vol.40Issue(3)：457-461,5.DOI:10.3724/SP.J.1096.2012.10683
膜基质免疫分析法同时检测玉米中伏马菌素B1和呕吐毒素
Simultaneous Detection of Fumonisin B1 and Deoxynivalenol in Corn Samples Using a Polyvinylidene Fluoride Membrane-based Immunoassay
摘要
Abstract
A Polyvinylidene fluoride (PVDF) membrane-based direct competition immunoassay for simultaneous detection of fumonisin Bi(FB1) and deoxynivalenol (DON) in corn samples was developed. PVDF membrane was soaked and activated in methanol, and then the conjugates (FB1-ovalbumin(OVA) and DON-cBSA) were spotted on the membrane reaction zone using the tip of dispenser. Horseradish peroxidase (HRP) was successfully coupled to anti-Fbi monoclonal antibody (McAb) and anti-DON McAb by cyanuric chloride method and periodate method respectively. Simultaneous detection of fumonisin B, and deoxynivalenol in one membrane in the system was carried out under direct competition immunoassay mode. The detection limits for fumonisins B1 and deoxynivalenol were 2. 5 and 50 μg/L, respectively. With very simple sample pre-treatment, a batch of extracted samples was analyzed within 15 min. The concentration and type of mycotoxins in samples were determined visibly by the naked eye. Fumonisins B1 and deoxynivalenol in 30 corn samples were screened by the PVDF membrane-based immunoassay, the results showed no significant difference with the ELISA kit.关键词
聚偏氟乙烯膜/伏马菌素B1/呕吐毒素/直接竞争免疫检测Key words
Polyvinylidene fluoride membrane/ Fumonisins B1/ Deoxynivalenol/ Direct competition immunoassay引用本文复制引用
康敏,许杨,何庆华,王丹..膜基质免疫分析法同时检测玉米中伏马菌素B1和呕吐毒素[J].分析化学,2012,40(3):457-461,5.基金项目
本文系国家“863”计划项目(No.2007AA102427)资助 （No.2007AA102427）
