分析化学2012，Vol.40Issue(4)：493-497,5.DOI:10.3724/SP.J.1096.2012.20119
新型微梁阵列生化传感器的研制
A Novel Microcantilever Array Biochemical Sensor
摘要
Abstract
To eliminate the impact of environmental noise such as temperature drift and change of liquid refractive index in the single microcantilever biochemical sensing system and achieve rapid parallel detection of a variety of target molecules, a novel microcantilever array biochemical sensor was designed and manufactured. When the microcantilever array was scanned by a piezoelectric-driven laser beam, the kinetic curves of the specific biochemical reaction on the microcantilever array surface was obtained by real-time monitoring the deflections of the microcantilevers with the optical lever read-out technique. In the experiment, the system optical stability was verified by 9 h scanning of two fixed points with 250 μm pitch, and also the system detection reliability was verified by the temperature excitation test, in which the microcantilever array deflections were basically consistent with each other (error 6. 5%) when heating up 6 ℃. Finally, 10 μg/L of clenbuterol antigen was detected by immobilizing clenbuterol antibody on the gold-coated side of the microcantilever array with the self-made capillary array modification device, which verified the practical feasibility of the microcantilever array sensor.关键词
生化传感器/无标记/微梁阵列/压电驱动/光杠杆法/毛细管阵列Key words
Biochemical sensor/Label-free/Microcantilever array/Piezoelectric-driven/Optical lever/Capillary array引用本文复制引用
邬林,周夏荣,吴尚犬,王萍,张青川,伍小平..新型微梁阵列生化传感器的研制[J].分析化学,2012,40(4):493-497,5.基金项目
本文系国家自然科学基金(No.11127201)资助 （No.11127201）
