分析化学2012，Vol.40Issue(4)：529-533,5.DOI:10.3724/SP.J.1096.2012.10911
高效液相色谱法同时测定食品中6种对羟基苯甲酸酯
Simultaneous Determination of Six Kinds of 4-Hydrobenzoic Acid Ester in Food Using High Performance Liquid Chromatography-Diode Array Detection
摘要
Abstract
A method was developed for the determination of preservatives of six 4-hydrobenzoic acid ester by HPLC-DAD. The samples of 4-hydrobenzoic acid ester were extracted by acetonitrile and purified by LC-C18 SPE. The analytes were separated on an Agilent C18 column, eluted by gradient with methanol and water, detected by diode array detector, and quantified by external standard method. The calibration curve was linear over the concentration range of 1. 0 - 500. 0 mg/L (r≥ 0. 9997). The lower limit of quantification in food was 0. 0016 - 0. 0081 mg/L. The relative recoveries of six kinds of 4-hydrobenzoic acid ester in vegetables and fruit of surface treatment, vinegar, carbonated beverages, milk, and food fillings were 85. 1% - 95. 4%, and relative standard deviations were 3.6% -13.0%.关键词
高效液相色谱法/对羟基苯甲酸酯/固相萃取/食品Key words
High performance liquid chromatography-diode array detection/4-Hydrobenzoic acid ester/Solid phase extract/Food引用本文复制引用
曹淑瑞,刘治勇,张雷,李贤良,王国民,唐柏彬,袁若..高效液相色谱法同时测定食品中6种对羟基苯甲酸酯[J].分析化学,2012,40(4):529-533,5.基金项目
本文系国家自然科学基金(No.20675064),重庆市自然科学基金(No.CSTC-2009BA1003),西南大学高新技术培育基金(No.XSGX02)项目资助 （No.20675064）
