分析化学2012，Vol.40Issue(4)：584-588,5.DOI:10.3724/SP.J.1096.2012.10618
电化学研究铝及其纳米Al13对谷胱甘肽还原酶活性的影响
Electrochemical Studies on Effects of Glutathione Reductase Activity by Al3+ and Nano-Al13
摘要
Abstract
We prepared a MWNTs/chitosan modified bare GCE that obviously decreased the overpo-tential of reduced coenzyme II NADPH from 700 mV at GCE to 380 mV at the modified electrode, meanwhile increased the catalytic current apparently. Oxidation current of NADPH is proportional to NADPH concentration in the range of 0.08 - 2.60 mmol/L with the lower detection limit for 18. 3 μmol/L. In the catalytic reaction of glutathione reductase(GR), we used the modified electrode to monitor the change of oxidation current of NADPH based on amperometic irt curve, the enzyme activity could be represented by the persisting decrease in the limiting steady-state oxidation current of NADPH. The effects of Al3+ and nano-Al13 on the activity of GR-catalyzed reactions under different pH values and different aluminum concentrations were studied. The results showed that the Al3+ and nano-Al13 could inhibit the activity of enzyme GR in aqueous solution.关键词
纳米Al13/谷胱甘肽还原酶/还原型辅酶Ⅱ/多壁碳纳米管/修饰电极Key words
Nano-Al13/Glutathione reductase/Reduced coenzyme II /Multi-walled carbon nanotubes/Modified electrode引用本文复制引用
张楠楠,汤勇铮,马菲,李卉卉,陆天虹,杨小弟..电化学研究铝及其纳米Al13对谷胱甘肽还原酶活性的影响[J].分析化学,2012,40(4):584-588,5.基金项目
本文系国家自然科学基金(Nos.20875047,20902048)和江苏高校优势学科建设工程资助 （Nos.20875047,20902048）
