高等学校化学学报2012，Vol.33Issue(3)：475-480,6.DOI:10.3969/j.issn.0251-0790.2012.03.008
高灵敏CdTe量子点探针的构建及与金属离子的作用
Construction of High-Sensitivity CdTe Quantum Dots as Fluorescence Probe and Its Interaction with Metal Ions
摘要
Abstract
We used denatured bovine serum albumin ( dBSA) to modify water-soluble CdTe quantum dots (QDs) , constructed the metal ion probe with high detection sensitivity, and studied its detection performance and mechanism for heavy metal ions. Water-soluble CdTe quantum dots with a high quantum yield and thiogly-colic acid(TGA) as stabilizer were synthesized by optimizing the reaction conditions. We used dBSA to modify the CdTe QDs of different sizes respectively, determined the optimum ratios of dBSA to QDs. And then the interaction between QDs and metal ions was studied after purifying the dBSA surface-modified QDs ( dBSA-QDs). Experimental results showed that the conjugation of QDs by dBSA efficiently improved the photolumi-nescence quantum yield, the chemical stability of QDs and stability against photobleaching; meanwhile the fluorescence of dBSA-QDs can be quenched by heavy metal ions effectively, and the detection sensitivity was improved greatly compared with TGA-coated QDs.关键词
碲化镉量子点/巯基乙酸/变性的牛血清白蛋白/金属离子Key words
Quantum dots ( QDs) /Thioglycolic acid ( TGA ) /Denatured bovine serum albumin ( dBSA ) /Metal ions分类
化学化工引用本文复制引用
程芳,刘贞,胡育筑,吴盛美,严拯宇..高灵敏CdTe量子点探针的构建及与金属离子的作用[J].高等学校化学学报,2012,33(3):475-480,6.基金项目
国家自然科学基金(批准号:30873197)、国家基础科学人才培养基金(批准号:J0630858)和省级大学生实践创新训练计划项目资助. （批准号:30873197）
