高等学校化学学报2012，Vol.33Issue(3)：516-520,5.DOI:10.3969/j.issn.0251-0790.2012.03.016
N-磷酰化多巴胺与溶菌酶相互作用的ESI-MS研究
Interaction Between N-Phosphoryl Dopamines and Lysozyme by ESI-MS
摘要
Abstract
The non-covalent interaction between a series of Iv-phosphoryl dopamines synthesized in our previous work and lysozyme was studied by electro spray ionization-mass spectroscopy ( ESI-MS). The effects of different groups of P on Iv-phosphoryl dopamine were compared. The results showed that the length of alkyl group of alkoxyl group on P was very important for the interaction under ESI-MS conditions. N-phosphoryl dopamines with longer carbon chains could contract the protein configuration in solution and were inclined to form complexes with lysozyme that have a lower charge and higher m/z and more stability. The sequence of carbon chain also had an obvious effect on the stability of the complex. Straight chain was more beneficial for the stability than branched chain when the number of carbon was equal. Isomers showed a different effect on the protein configuration in solution when alkyl group of alkoxyl group on P was propyl or isopropyl group. The maximal stoichiometric proportion of the complexes of N-phosphoryl dopamines and lysozyme is 5:1. The results are valuable for the design of new active dopamine molecules.关键词
电喷雾离子肼-质谱/N-磷酰化多巴胺/溶菌酶/非共价相互作用Key words
Electro spray ionization-mass spectroscopy (ESI-MS) /N-Phosphoryl dopamine/Lysozyme/Non-covalent interaction分类
化学化工引用本文复制引用
武现丽,韩国胜,王珍,曹书霞,廖新成..N-磷酰化多巴胺与溶菌酶相互作用的ESI-MS研究[J].高等学校化学学报,2012,33(3):516-520,5.基金项目
国家自然科学基金(批准号:20672104)资助. （批准号:20672104）
