高等学校化学学报2012，Vol.33Issue(6)：1177-1181,5.DOI:10.3969/j.issn.0251-0790.2012.06.011
基于表面等离子体共振技术用鸡蛋黄抗体IgY测定人血清中转铁蛋白
Determination of Transferrin in Human Serum Samples Using Chicken Egg Yolk Antibodies(IgY) by Surface Plasmon Resonance
摘要
Abstract
Chicken egg yolk antibodies ( IgY) instead of traditional mammalian antibodies were immobilized to a sensor chip CM5 for the detection of transferrin in human serum by surface plasmon resonance ( SPR). The conditions for immobilizing chicken anti -human transferring antibodies and regeneration were studied. Chicken egg yolk antibodies were immobilized under the condition of a -flow rate of 5μL/min, pH = 4. 0 and the concentration of 100μg/mL. The good linear range was obtained over 50-500 ng/mL, the detection limit was 39. 56 ng/mL. The determination of transferrin in human serum showed good reproduction and precision, intra-day CV was below 5% , and inter-day CV was below 8% , average recoveries ranged from 86. 22% to 94.51%.关键词
表面等离子体共振/鸡蛋黄抗体/人血清/转铁蛋白Key words
Surface plasmon resonance/Chicken egg yolk antibody(IgY)/Human serum/Transferrin分类
化学化工引用本文复制引用
王春宇,冯锋,白云峰,陈泽忠,田茂忠,邱建丁,倪永年..基于表面等离子体共振技术用鸡蛋黄抗体IgY测定人血清中转铁蛋白[J].高等学校化学学报,2012,33(6):1177-1181,5.基金项目
国家自然科学基金(批准号:21175085)和山西省自然科学基金(批准号:2009011015-1)资助. （批准号:21175085）
