高等学校化学学报2012，Vol.33Issue(6)：1195-1204,10.DOI:10.3969/j.issn.0251-0790.2012.06.014
配体对CdTe量子点与BSA的选择性相互作用的影响
Ligand Effects on Selective Interaction of CdTe Quantum Dots with BSA
摘要
Abstract
Five water-soluble CdTe quantum dots ( QDs) were synthesized using thioglycolic acid ( TGA) , 3-mercaptopropionic acid( MPA) , l-thioglycerol(TG), L-cysteine(L-cys) and glutathione ( GSH) as stabilizers, respectively. The interaction of CdTe QDs with bovine serum albumin (BSA) was studied by UV-Vis absorption and photoluminescence spectroscopy. Experimental results demonstrated that fluorescence of BSA could be markedly quenched by all these five CdTe QDs in Tris-HCl buffer solution ( pH = 7. 40). The quenching ability of CdTe QDs to BSA is as GSH>Z,-cys>TGA>TG>MPA in the order of ligands. However, when BSA was added into CdTe QDs solution, five CdTe QDs exhibited apparently selective responses to BSA. As the concentration of BSA increased, fluorescence intensity of CdTe QDs capped by TGA and MPA were both significantly enhanced firstly, then declined sharply. Under the same experimental conditions, fluorescence intensity of L-cys-capped CdTe QDs was significantly quenched by BSA, and that of TG-capped CdTe QDs was only slightly quenched by BSA. While almost no change of GSH-capped QDs fluorescence was observed in the presence of BSA. These results indicated that ligand molecular structure of CdTe QDs played an important role in the interaction between BSA and CdTe QDs. Time-resolved luminescence dynamics suggested that the ground state complex of BSA and CdTe QDs could form by electrostatic interaction. Possible interaction mechanism of BSA with CdTe QDs was proposed based on the molecular structure of ligands.关键词
CdTe量子点/配体影响/牛血清白蛋白/选择性相互作用/光致发光Key words
CdTe quantum dots(QDs)/Ligand effect/Bovine serum albumin(BSA)/Selective interaction/Photoluminescence分类
化学化工引用本文复制引用
王珊珊,王雪婷,郭明明,于俊生..配体对CdTe量子点与BSA的选择性相互作用的影响[J].高等学校化学学报,2012,33(6):1195-1204,10.基金项目
国家自然科学基金(批准号:20875045)、国家自然科学基金委员会创新研究群体科学基金(批准号:20821063)和国家"九七三"计划项目(批准号:2010CB732401)资助. （批准号:20875045）
